Plasmid_Backbone
pGADT7-GRI

Part:BBa_K3978002

Designed by: Ding Haote   Group: iGEM21_HiZJU-China   (2021-10-21)


It is a plasmid which can express a fusion protein of AD and GRIP 1.

It is a plasmid which can express a fusion protein of AD and GRIP 1. Coding sequence of target gene was inserted into an expression vectors with GRIP 1 and Plasmid(BBa_K3978000) to obtain BBa_K3978002. We transformed the constructed plasmid into yeast Y2H to construct yeast two-hybrid technology system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal EcoRI site found at 7996
    Illegal XbaI site found at 9622
    Illegal XbaI site found at 10678
    Illegal XbaI site found at 11047
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal EcoRI site found at 7996
    Illegal NheI site found at 4496
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
    Illegal NotI site found at 4517
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal EcoRI site found at 7996
    Illegal BglII site found at 1898
    Illegal BglII site found at 8272
    Illegal BglII site found at 10357
    Illegal BglII site found at 10402
    Illegal BglII site found at 10450
    Illegal BglII site found at 10633
    Illegal BglII site found at 10669
    Illegal BamHI site found at 2018
    Illegal XhoI site found at 2030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal EcoRI site found at 7996
    Illegal XbaI site found at 9622
    Illegal XbaI site found at 10678
    Illegal XbaI site found at 11047
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1990
    Illegal EcoRI site found at 7996
    Illegal XbaI site found at 9622
    Illegal XbaI site found at 10678
    Illegal XbaI site found at 11047
    Illegal PstI site found at 8
    Illegal PstI site found at 2036
    Illegal PstI site found at 4506
    Illegal NgoMIV site found at 210
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 860
    Illegal BsaI site found at 10517
    Illegal BsaI.rc site found at 5714
    Illegal SapI site found at 4631


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